Crown ether-mediated extraction and functional conversion of cytochrome c in ionic liquids

Kojiro Shimojo, Kazunori Nakashima, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

We report that a macrocyclic ligand enables transfer of a protein from an aqueous phase to ionic liquids. The extraction behavior of heme protein cytochrome c (Cyt-c) from an aqueous phase into ionic liquids was investigated with crown ethers. A hydroxyl-group-containing ionic liquid with dicyclohexano-18-crown-6 was found to be capable of quantitative partitioning of Cyt-c, whereas the protein transfer using conventional organic solvents was negligibly small. Furthermore, we clarified that Cyt-c solubilized in ionic liquids caused a structural transformation of Cyt-c, which triggers its functional conversion from an electron-transfer protein to peroxidase.

Original languageEnglish
Pages (from-to)2-5
Number of pages4
JournalBiomacromolecules
Volume7
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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