Cross-Regulation of an Artificial Metalloenzyme

Yasunori Okamoto; Thomas R. Ward

doi:10.1002/anie.201702181

Cross-Regulation of an Artificial Metalloenzyme

Yasunori Okamoto, Thomas R. Ward

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Cross-regulation of complex biochemical reaction networks is an essential feature of living systems. In a biomimetic spirit, we report on our efforts to program the temporal activation of an artificial metalloenzyme via cross-regulation by a natural enzyme. In the presence of urea, urease slowly releases ammonia that reversibly inhibits an artificial transfer hydrogenase. Addition of an acid, which acts as fuel, allows to maintain the system out of equilibrium.

Original language	English
Pages (from-to)	10156-10160
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	56
Issue number	34
DOIs	https://doi.org/10.1002/anie.201702181
Publication status	Published - 2017 Aug 14
Externally published	Yes

Keywords

artificial metalloenzymes
biocatalysis
cross-regulation
hydrogenation
urease

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201702181

Cite this

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abstract = "Cross-regulation of complex biochemical reaction networks is an essential feature of living systems. In a biomimetic spirit, we report on our efforts to program the temporal activation of an artificial metalloenzyme via cross-regulation by a natural enzyme. In the presence of urea, urease slowly releases ammonia that reversibly inhibits an artificial transfer hydrogenase. Addition of an acid, which acts as fuel, allows to maintain the system out of equilibrium.",

keywords = "artificial metalloenzymes, biocatalysis, cross-regulation, hydrogenation, urease",

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note = "Funding Information: Generous support from the Swiss National Science Foundation (grant 200020_162348) and from the NCCR Molecular Systems Engineering, an advanced ERC grant (DrEAM agreement number 694424) as well as a JSPS Overseas research fellowship to Y.O. is gratefully acknowledged. We thank Dr. M. D{\"u}rrenberger, Dr. V. K{\"o}hler and Dr. J. Rebelein for enlightening discussions and Umicore for a loan of [Cp*IrCl2]2. Publisher Copyright: {\textcopyright} 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.",

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AU - Ward, Thomas R.

N1 - Funding Information: Generous support from the Swiss National Science Foundation (grant 200020_162348) and from the NCCR Molecular Systems Engineering, an advanced ERC grant (DrEAM agreement number 694424) as well as a JSPS Overseas research fellowship to Y.O. is gratefully acknowledged. We thank Dr. M. Dürrenberger, Dr. V. Köhler and Dr. J. Rebelein for enlightening discussions and Umicore for a loan of [Cp*IrCl2]2. Publisher Copyright: © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

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KW - hydrogenation

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Cross-Regulation of an Artificial Metalloenzyme

Abstract

Keywords

ASJC Scopus subject areas

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