Cross-reactivity of murine monoclonal anti-DNA antibodies with human and murine skin: A possible pathogenetic role in skin lesions of lupus erythematosus

Setsuya Aiba, Osamu Yoshie, Yasushi Tomita, Hachiro Tagami

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Anti-DNA autoantibodies are known to cross-react with a wide variety of substances including cell-surface molecules. Thus, we examined cross-reactivities of 20 murine monoclonal anti-DNA antibodies with normal human and mouse skin tissues. Hybridomas producing these monoclonal antibodies were established from non-immunized spleen cells from autoimmune MRL-1 pr/1 pr mice, a strain characterized by spontaneous development of SLE-like disorders including skin changes. They were selected based on their reactivity to DNA in a typical enzyme-linked immunosorbent assay, in which nine monoclonal antibodies were reactive with both double-stranded DNA and single-stranded DNA, whereas nine monoclonals were reactive only with single-stranded DNA. Even though only seven of them were observed to stain nuclei, most of the monoclonal antibodies revealed strong and distinct cross-reactivities to various components of the skin tissues including the epidermal basement membrane, keratinocytes at different locations of the epidermis, melanocytes, Langerhans cells, Thy-1+ dendritic cells in the case of murine skin, and mast cells. Our results suggest a possible role of so-called anti-DNA antibodies with high or low affinities to DNA in the pathogenesis of cutaneous lesions of lupus erythematosus.

Original languageEnglish
Pages (from-to)739-745
Number of pages7
JournalJournal of Investigative Dermatology
Volume93
Issue number6
DOIs
Publication statusPublished - 1989 Dec

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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