CrkL is a novel target of Sprouty2 in fibroblast growth factor signaling

Takayuki Satoh, Satoru Torii, Kei Nakayama, Eisuke Nishida

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Sprouty, an inhibitor of receptor tyrosine kinase signaling, plays an important role in the regulation of a wide variety of biological processes. Although it is established that the Sprouty inhibitory activity is induced by tyrosine phosphorylation in response to stimuli, its action mechanisms have not been fully elucidated. Here, we report identification of a novel target of Sprouty. We find that Sprouty1 and Sprouty2 bind to the adaptor protein CrkL in a stimulus-dependent manner. Biochemical analyses show that the binding requires tyrosine phosphorylation of Sprouty and that both the SH2 domain and the N-terminal SH3 domain of CrkL are necessary for the binding. In fibroblast growth factor-stimulated NIH3T3 cells, CrkL binding to Sprouty2 occurs concomitantly with tyrosine phosphorylation of Sprouty2, which occurs slowly but is sustained. Importantly, our results show that tyrosine-phosphorylated Sprouty2 suppresses Rap1 activation. These results taken together indicate that Sprouty2 acts as an inhibitor of CrkL-Rap1 signaling.

    Original languageEnglish
    Pages (from-to)161-168
    Number of pages8
    JournalGenes to Cells
    Volume15
    Issue number3
    DOIs
    Publication statusPublished - 2010 Mar

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

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