Critical Roles of TRAF2 and TRAF5 in Tumor Necrosis Factor-induced NF-κB Activation and Protection from Cell Death

Kurisu Tada; Tatsuma Okazaki; Sachiko Sakon; Tomonari Kobarai; Kyoko Kurosawa; Shoji Yamaoka; Hiroshi Hashimoto; Tak Wah Mak; Hideo Yagita; Ko Okumura; Wen Chen Yeh; Hiroyasu Nakano

doi:10.1074/jbc.M104837200

Critical Roles of TRAF2 and TRAF5 in Tumor Necrosis Factor-induced NF-κB Activation and Protection from Cell Death

Kurisu Tada, Tatsuma Okazaki, Sachiko Sakon, Tomonari Kobarai, Kyoko Kurosawa, Shoji Yamaoka, Hiroshi Hashimoto, Tak Wah Mak, Hideo Yagita, Ko Okumura, Wen Chen Yeh, Hiroyasu Nakano

Research output: Contribution to journal › Article › peer-review

248 Citations (Scopus)

Abstract

Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) were identified as signal transducers for the TNF receptor superfamily. However, the exact roles of TRAF2 and TRAF5 in TNF-induced NF-κB activation still remain controversial. To address this issue, we generated TRAF2 and TRAF5 double knockout (DKO) mice. TNF- but not interleukin-1-induced nuclear translocation of NF-κB was severely impaired in murine embryonic fibroblasts (MEFs) derived from DKO mice. Moreover, DKO MEFs were more susceptible to TNF-induced cytotoxicity than TRAF2 knockout MEFs. Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF-induced NF-κB activation and protection from cell death.

Original language	English
Pages (from-to)	36530-36534
Number of pages	5
Journal	Journal of Biological Chemistry
Volume	276
Issue number	39
DOIs	https://doi.org/10.1074/jbc.M104837200
Publication status	Published - 2001 Sep 28
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Critical Roles of TRAF2 and TRAF5 in Tumor Necrosis Factor-induced NF-κB Activation and Protection from Cell Death. / Tada, Kurisu; Okazaki, Tatsuma; Sakon, Sachiko; Kobarai, Tomonari; Kurosawa, Kyoko; Yamaoka, Shoji; Hashimoto, Hiroshi; Mak, Tak Wah; Yagita, Hideo; Okumura, Ko; Yeh, Wen Chen; Nakano, Hiroyasu.

In: Journal of Biological Chemistry, Vol. 276, No. 39, 28.09.2001, p. 36530-36534.

Research output: Contribution to journal › Article › peer-review

Tada, Kurisu ; Okazaki, Tatsuma ; Sakon, Sachiko ; Kobarai, Tomonari ; Kurosawa, Kyoko ; Yamaoka, Shoji ; Hashimoto, Hiroshi ; Mak, Tak Wah ; Yagita, Hideo ; Okumura, Ko ; Yeh, Wen Chen ; Nakano, Hiroyasu. / Critical Roles of TRAF2 and TRAF5 in Tumor Necrosis Factor-induced NF-κB Activation and Protection from Cell Death. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 39. pp. 36530-36534.

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abstract = "Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) were identified as signal transducers for the TNF receptor superfamily. However, the exact roles of TRAF2 and TRAF5 in TNF-induced NF-κB activation still remain controversial. To address this issue, we generated TRAF2 and TRAF5 double knockout (DKO) mice. TNF- but not interleukin-1-induced nuclear translocation of NF-κB was severely impaired in murine embryonic fibroblasts (MEFs) derived from DKO mice. Moreover, DKO MEFs were more susceptible to TNF-induced cytotoxicity than TRAF2 knockout MEFs. Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF-induced NF-κB activation and protection from cell death.",

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AU - Kobarai, Tomonari

AU - Kurosawa, Kyoko

AU - Yamaoka, Shoji

AU - Hashimoto, Hiroshi

AU - Mak, Tak Wah

AU - Yagita, Hideo

AU - Okumura, Ko

AU - Yeh, Wen Chen

AU - Nakano, Hiroyasu

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AB - Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) were identified as signal transducers for the TNF receptor superfamily. However, the exact roles of TRAF2 and TRAF5 in TNF-induced NF-κB activation still remain controversial. To address this issue, we generated TRAF2 and TRAF5 double knockout (DKO) mice. TNF- but not interleukin-1-induced nuclear translocation of NF-κB was severely impaired in murine embryonic fibroblasts (MEFs) derived from DKO mice. Moreover, DKO MEFs were more susceptible to TNF-induced cytotoxicity than TRAF2 knockout MEFs. Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF-induced NF-κB activation and protection from cell death.

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Critical Roles of TRAF2 and TRAF5 in Tumor Necrosis Factor-induced NF-κB Activation and Protection from Cell Death

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