TY - GEN
T1 - Creation of DNA microdroplets based on phase transition and sequence design
AU - Sato, Yusuke
AU - Sakamoto, Tetsuro
AU - Takinoue, Masahiro
N1 - Funding Information:
This research was supported by JSPS KAKENHI to M.T. (No. JP17H01813, JP18K19834) and Y.S. (No. JP18J00720), Research Encouragement Grants from The Asahi Glass Foundation to M.T., and Support for Tokyotech Advanced Researchers to M.T. Y.S. is a JSPS Research Fellow (SPD).
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - DNA is a promising materials to design, construct, and control micrometer-sized structures. A few studies have focused on the phase transition of DNA microstructures. However, there is less knowledge of the effects of the base sequence of DNA on the phase behavior. Here, we reports DNA microdroplets that is a novel and unique DNA microstructure. We designed sequences of DNAs and successfully visuzalized the formation of DNA microdroplets via phase transition. We believe that our results provide new aspects of DNA as a soft/fluid material, which would contribute to various fields such as bioengineering, microfluidics, and artificial cell engineering.
AB - DNA is a promising materials to design, construct, and control micrometer-sized structures. A few studies have focused on the phase transition of DNA microstructures. However, there is less knowledge of the effects of the base sequence of DNA on the phase behavior. Here, we reports DNA microdroplets that is a novel and unique DNA microstructure. We designed sequences of DNAs and successfully visuzalized the formation of DNA microdroplets via phase transition. We believe that our results provide new aspects of DNA as a soft/fluid material, which would contribute to various fields such as bioengineering, microfluidics, and artificial cell engineering.
KW - Biomaterials
KW - DNA nanotechnology
KW - Droplets
KW - Phase transition
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M3 - Conference contribution
AN - SCOPUS:85094941645
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 16
EP - 17
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -