Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

Seiji Sakamoto, Mika Terauchi, Anna Hugo, Tanner Kim, Yasuyuki Araki, Takehiko Wada

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is “covalently” cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.

Original languageEnglish
Pages (from-to)10323-10325
Number of pages3
JournalChemical Communications
Volume49
Issue number87
DOIs
Publication statusPublished - 2013 Oct 8

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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