Cre-loxP-mediated bax gene activation reduces growth rate and increases sensitivity to chemotherapeutic agents in human gastric cancer cells

Koga Komatsu, Susumu Suzuki, Tooru Shimosegawa, Jun Ichi Miyazaki, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Dysregulation of apoptosis may be closely related to the development of cancer and its chemoresistance. Overexpression of Bax, an inducer of apoptosis, has led to increased cell death in a variety of cancer cell lines. In this study, we investigated the effect of Bax overexpression in two gastric cancer cell lines, MKN-28 and MKN-45, using a Cre-loxP-mediated inducible expression system. After induction of bax, both cell lines showed decreased proliferation, partially due to increased cell death. Furthermore, Bax-expressing MKN-28 cells were more sensitive to cisplatin. These results indicate that up-regulation of the bax gene may provide a novel strategy for the treatment of gastric cancer.

Original languageEnglish
Pages (from-to)885-892
Number of pages8
JournalCancer Gene Therapy
Volume7
Issue number6
DOIs
Publication statusPublished - 2000

Keywords

  • Apoptosis
  • Bax
  • Bcl-2
  • Chemotherapy
  • Cre-IoxP system

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

Fingerprint Dive into the research topics of 'Cre-loxP-mediated bax gene activation reduces growth rate and increases sensitivity to chemotherapeutic agents in human gastric cancer cells'. Together they form a unique fingerprint.

Cite this