COX-2 induces T cell accumulation and IFN-γ production during the development of chromium allergy

Ratri Maya Sitalaksmi, Koyu Ito, Kouetsu Ogasawara, Yoshiko Suto, Madoka Itabashi, Kyosuke Ueda, Noriyasu Hirasawa, Takayuki Narushima, Nike Hendrijantini, Utari Kresnoadi, Keiichi Sasaki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Chromium (Cr) is commonly added into various metal alloys to improve some mechanical properties such as corrosion resistance, strength, and workability. However, Cr is also known to be a metal allergen for some individuals. Metal allergy is a T cell-mediated disease with symptoms of inflammation and swelling that involve inflammatory cytokines and prostaglandins. Hence, suppressing these inflammation paths by using COX-2 inhibitor might be useful in treating Cr allergy. In this study, mice were used with Cr-induced allergy challenge model. The mice were injected with celecoxib once per day for 7 days one hour after the challenge. Footpad samples were stained with haematoxylin and eosin (H&E), and lymphocytes were isolated from popliteal lymph nodes for the flow cytometric analysis. The results show that both prostaglandin E2 (PGE2), a known mediator of inflammation, and cyclooxygenases (COX)-2 have important roles in the development of Cr allergy. Further, COX-2 inhibitor, celecoxib, was effective in relieving swelling and inflammation in Cr-allergic mice concordant with suppression of IFN-γ production by CD8+ T cells and T cell accumulation in the lymph nodes. Therefore, the inhibition of COX-2 may be a therapeutic target for Cr allergy, and additional molecules in the PGE2 signalling pathway may also be an effective therapeutic target for the treatment of metal allergy.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalAutoimmunity
Volume52
Issue number5-6
DOIs
Publication statusPublished - 2019 Aug 18

Keywords

  • COX-2
  • Metal allergy
  • celecoxib
  • chromium
  • inflammation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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