Covalent protein labeling based on noncatalytic β-Lactamase and a designed FRET substrate

Shin Mizukami, Shuji Watanabe, Yuichiro Hori, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

115 Citations (Scopus)

Abstract

Techniques for labeling proteins with small molecules have attracted the attention of many life scientists. We have developed a novel protein labeling system that combines a genetically modified, noncatalytic β-lactamase variant and specific mechanism-based fluorescent probes. Rational design of the tag protein and the labeling probes enables highly specific incorporation of the fluorogen. The feasibility of our approach was confirmed by gel electrophoresis, mass spectrometry, fluorescence spectroscopy, and fluorescence microscopic imaging. Labeling techniques that satisfy the dual criteria of specificity and fluorogenicity have rarely been reported. As a consequence, this method could be a broadly useful research tool in the field of life science.

Original languageEnglish
Pages (from-to)5016-5017
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number14
DOIs
Publication statusPublished - 2009 Apr 15
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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