Corticotropin-Releasing Hormone in the Human Hypothalamus. Free-Floating Immunostaining Method

Kazuhiro Takahashi, Nobuaki Sasano, Toraichi Mouri, Keiichi Itoi, Osamu Murakami, Kaoru Yoshinaga, Teiji Yamamoto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We have clearly demonstrated corticotropin-releasing hormone (CRH) immunoreactive cell bodies and nerve fibers in the human hypothalamus by immunocytochemistry using free-floating sections instead of paraffin-embedded sections. Human hypothalami were obtained at autopsy, fixed and cryostat-sectioned at 40 microns. Free-floating sections were immunostained with antibody to CRH using the Vector ABC system. Most of CRH immunoreactive nerve fibers from the paraventricular nucleus pass under the fornix, while some CRH immunoreactive nerve fibers pass beyond the fornix and some through the fornix. Then the CRH immunoreactive nerve fibers run downward, medially to the supraoptic nucleus and toward the pituitary stalk. This method of immunocytochemistry is a very sensitive and suitable means for immunocytochemical studies of neuropeptides in the human brain.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalEndocrinologia Japonica
Volume36
Issue number2
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Endocrinology
  • Engineering(all)

Fingerprint Dive into the research topics of 'Corticotropin-Releasing Hormone in the Human Hypothalamus. Free-Floating Immunostaining Method'. Together they form a unique fingerprint.

Cite this