Correlative polymorphism of NAD(P)H: Quinone oxidoreductase (NQO1) with telomere shortening in colorectal cancer

Sho Takagi, Yoshitaka Kinouchi, Nobuo Hiwatashi, Masashi Hirai, Susumu Suzuki, Seiichi Takahashi, Kenichi Negoro, Nobuya Obana, Tooru Shimosegawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Aim: The aim of this study was to examine whether any relationships could be found among polymorphism of the NQO1 gene, telomere length and telomerase activity in colorectal cancers. Materials and Methods: Fifty-one invasive colorectal cancers were studied. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was undergone to detect mutation of the NQO1 gene. Telomere length was examined by Southern blot analysis. Telomerase activity was assayed by telomeric repeat amplification protocol with minor modifications. Results: Of the 51 tumors, 20 (39.2%) and 9 (17.6%) were heterozygous and homozygous for the mutation, respectively. Most of the cases homozygous for the mutation (88.9%) showed short telomeres and its frequency was significantly higher than in those heterozygous (p=0.0432). However no relationship was found between the telomerase activity and mutation in the NQO1 gene. Conclusion: Our data suggest that oxidative stress by the lack of NQO1 activity could result in telomere shortening through colorectal cancinogenesis.

Original languageEnglish
Pages (from-to)2749-2752
Number of pages4
JournalAnticancer research
Volume22
Issue number5
Publication statusPublished - 2002 Sep

Keywords

  • Colorectal cancer
  • DT-diaphorase
  • NAD(P)H: quinone oxidoreductase
  • NQO1
  • Polymorphism
  • Telomerase
  • Telomere

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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