Correlation of differential expression of silkworm antimicrobial peptide genes with different amounts of rel family proteins and their gene transcriptional activity

Hiromitsu Tanaka, Aki Sagisaka, Yoshiro Nakajima, Kosuke Fujita, Shigeo Imanishi, Minoru Yamakawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In the silkworm, Bombyx mori, antimicrobial peptide (AMP) genes are upregulated in the larval fat body by injection of bacteria and peptidoglycans (PGNs). The DAP-type PGN from Escherichia coli and Bacillus subtilis exhibited stronger elicitor activity for expression of AMP genes in B. mori than Lys-type PGN from Staphylococcus aureus, suggesting that differences in bacterial influence on the induction levels of these genes depend on the differences in types of PGN. BmRelish1 mRNA was more abundant than BmRel mRNAs in the larval fat bodies. Moreover, the ability of the BmRelish1 active form to enhance the promoter activity of AMP genes was higher than that of BmRels. The difference was related to the binding affinity of Rel family proteins to kB sites. Our results suggest that different amounts and different transcriptional activities of Rel family proteins result in differential activation of AMP genes by PGN type and bacterium species.

Original languageEnglish
Pages (from-to)599-606
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number3
DOIs
Publication statusPublished - 2009 Apr 30
Externally publishedYes

Keywords

  • Antimicrobial peptide genes
  • Bombyx mori
  • Insect immunity
  • Peptidoglycan
  • Rel family proteins

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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