Correlation of Denitrifying Capability with the Existence of nap, nir, nor and nos Genes in Diverse Strains of Soybean Bradyrhizobia

Reiko Sameshima-Saito, Kaori Chiba, Kiwamu Minamisawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To clarify the genetic basis to the diversity of denitrifying ability in soybean bradyrhizobia, we compared the end-products of denitrification (N2, N2O and NO2-) with the existence of denitrifying genes (napA, nirK, norCB and nosZ) of sixty phylogenetically diverse strains of Bradyrhizobium japonicum and B. elkanii. The results indicate that the existence of denitrifying genes directly determines phenotype (end-products) in most strains of B. japonicum. The denitrifying capability and gene set were reflected by phylogenetic position based on repeated sequences (RS)-fingerprints and 16S rRNA gene sequences. However, the denitrifying genes in HRS (highly repeated sequence-possessing) strains of B. japonicum, which were identified based on RS-fingerprints as having heavy hybridization, resulted in an inconsistent correlation probably because of genomic rearrangements. The evolutionary and ecological implications of the denitrifying genes and capability in soybean bradyrhizobia are discussed.

Original languageEnglish
Pages (from-to)174-184
Number of pages11
JournalMicrobes and environments
Volume21
Issue number3
DOIs
Publication statusPublished - 2006 Jan 1

Keywords

  • Bradyrhizobium
  • denitrification
  • denitrifying genes
  • nosZ

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

Fingerprint Dive into the research topics of 'Correlation of Denitrifying Capability with the Existence of nap, nir, nor and nos Genes in Diverse Strains of Soybean Bradyrhizobia'. Together they form a unique fingerprint.

  • Cite this