TY - JOUR
T1 - Correlation between loss of PTEN expression and Akt phosphorylation in endometrial carcinoma
AU - Kanamori, Y.
AU - Kigawa, J.
AU - Itamochi, H.
AU - Shimada, M.
AU - Takahashi, M.
AU - Kamazawa, S.
AU - Sato, S.
AU - Akeshima, R.
AU - Terakawa, N.
PY - 2001/8/15
Y1 - 2001/8/15
N2 - The tumor suppressor PTEN acts as a lipid phosphatase, regulates the phosphatidylinositol 3-kinase (PI3K)/Akt-signaling pathway, and modulates cell cycle progression and cell survival. Somatic mutations of PTEN have been reported in a variety of cancers, especially in endometrial carcinoma. To clarify whether and how PTEN and the PI3K/Akt pathway relates to endometrial carcinoma, we examined the expression of those pathway-related proteins in patients with endometrial carcinoma. Of 103 endometrial carcinomas, 37 (36%) showed negative immunohistochemical staining of PTEN. Western blotting revealed that the expression of PTEN in PTEN-negative cases was significantly lower compared with that in positive cases. In contrast, phospho-Akt level in negative cases was significantly higher. We found a significant inverse correlation between PTEN and phospho-Akt (r = -0.796). The expression of phospho-Bad was greater in negative cases, suggesting that Bad might be a target for Akt. The present study demonstrates the phosphorylation of Akt accompanied by the loss of PTEN in clinical specmens of endometrial carcinomas.
AB - The tumor suppressor PTEN acts as a lipid phosphatase, regulates the phosphatidylinositol 3-kinase (PI3K)/Akt-signaling pathway, and modulates cell cycle progression and cell survival. Somatic mutations of PTEN have been reported in a variety of cancers, especially in endometrial carcinoma. To clarify whether and how PTEN and the PI3K/Akt pathway relates to endometrial carcinoma, we examined the expression of those pathway-related proteins in patients with endometrial carcinoma. Of 103 endometrial carcinomas, 37 (36%) showed negative immunohistochemical staining of PTEN. Western blotting revealed that the expression of PTEN in PTEN-negative cases was significantly lower compared with that in positive cases. In contrast, phospho-Akt level in negative cases was significantly higher. We found a significant inverse correlation between PTEN and phospho-Akt (r = -0.796). The expression of phospho-Bad was greater in negative cases, suggesting that Bad might be a target for Akt. The present study demonstrates the phosphorylation of Akt accompanied by the loss of PTEN in clinical specmens of endometrial carcinomas.
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M3 - Article
C2 - 11309338
AN - SCOPUS:0034906660
VL - 7
SP - 892
EP - 895
JO - Clinical Cancer Research
JF - Clinical Cancer Research
SN - 1078-0432
IS - 4
ER -
