Correlation between depression of catabolite control of xylose metabolism and a defect in the phosphoenolpyruvate:Mannose phosphotransferase system in Pediococcus halophilus

K. Abe, K. Uchida

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pediococcus halophilus X-160 which lacks catabolite control by glucose was isolated from nature (soy moromi mash). Wild-type strains, in xylose-glucose medium, utilized glucose preferentially over xylose and showed diauxic growth. With wild-type strain I-13, xylose isomerase activity was not induced until glucose was consumed from the medium. Strain X-160, however, utilized xylose concurrently with glucose and did not show diauxic growth. In this strain, xylose isomerase was induced even in the presence of glucose. Glucose transport activity in intact cells of strain X-160 was <10% of that assayed in strain I-13. Determination of glycolytic enzymes did not show any difference responsible for the unique behavior of strain X-160, but the rate of glucose-6-phosphate formation with phosphoenolpyruvate (PEP) as a phosphoryl donor in permeabilized cells was <10% of that observed in the wild type. Starved P. halophilus I-13 cells contained the glycolytic intermediates 3-phosphoglycerate, 2-phosphoglycerate, and PEP (PEP pool). These were consumed concomitantly with glucose or 2-deoxyglucose uptake but were not consumed with xylose uptake. The glucose transport system in P. halophilus was identified as a PEP:mannose phosphotransferase system on the basis of the substrate specificity of PEP pool-starved cells. It is concluded that, in P. halophilus, this system is functional as a main glucose transport system and that defects in this system may be responsible for the depression of glucose-mediated catabolite control.

Original languageEnglish
Pages (from-to)1793-1800
Number of pages8
JournalJournal of bacteriology
Volume171
Issue number4
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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