TY - JOUR
T1 - Correlation between chromatin association and transcriptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae
AU - Harata, Masahiko
AU - Zhang, Yan
AU - Stillman, David J.
AU - Matsui, Daisuke
AU - Oma, Yukako
AU - Nishimori, Katsuhiko
AU - Mochizuki, Ryo
N1 - Funding Information:
We thank Dr Eiko Tsuchiya for plasmids. We gratefully acknowledge Dr Ulrike Wintersberger and Irene Görzer for helpful discussions of the results. This work was supported by a Grant-in-Aid for Scientific Research (11760051) from the Ministry of Education, Science, Sport, and Culture, Japan, and by the Kato Memorial Bioscience Foundation.
PY - 2002/4/15
Y1 - 2002/4/15
N2 - Actin-related proteins (Arps), which share a basal structure with actin but have distinct functions, have been found in a wide variety of organisms. While their functions are not yet clear, some Arps are localized in the nucleus and are suggested to contribute to the regulation of transcription. An essential gene of Saccharomyces cerevisiae, Act3p/Arp4, encodes the first identified nuclear Arp, which has been shown to bind to core histones in vitro. Here we have analyzed the in vivo function of Act3p/Arp4 on the his4-912δ promoter. Chromatin immunoprecipitation assays show that Act3p/Arp4 is bound to the entire his4-912δ promoter region. Conditional act3/arp4 mutations affect transcription from the his4-912δ promoter, where decreased Act3p/Arp4 binding and a change in nuclease sensitivity of chromatin were observed, showing the involvement of Act3p/Arp4 in the regulation of gene expression through the organization of chromatin structure. Taken together with the presence of Act3p/Arp4 in chromatin remodeling and histone acetyltransferase complexes, it is suggested that Act3p/Arp4 functions in transcriptional regulation to recruit chromatin remodeling and histone acetyl-transferase complexes onto chromatin.
AB - Actin-related proteins (Arps), which share a basal structure with actin but have distinct functions, have been found in a wide variety of organisms. While their functions are not yet clear, some Arps are localized in the nucleus and are suggested to contribute to the regulation of transcription. An essential gene of Saccharomyces cerevisiae, Act3p/Arp4, encodes the first identified nuclear Arp, which has been shown to bind to core histones in vitro. Here we have analyzed the in vivo function of Act3p/Arp4 on the his4-912δ promoter. Chromatin immunoprecipitation assays show that Act3p/Arp4 is bound to the entire his4-912δ promoter region. Conditional act3/arp4 mutations affect transcription from the his4-912δ promoter, where decreased Act3p/Arp4 binding and a change in nuclease sensitivity of chromatin were observed, showing the involvement of Act3p/Arp4 in the regulation of gene expression through the organization of chromatin structure. Taken together with the presence of Act3p/Arp4 in chromatin remodeling and histone acetyltransferase complexes, it is suggested that Act3p/Arp4 functions in transcriptional regulation to recruit chromatin remodeling and histone acetyl-transferase complexes onto chromatin.
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U2 - 10.1093/nar/30.8.1743
DO - 10.1093/nar/30.8.1743
M3 - Article
C2 - 11937627
AN - SCOPUS:0037089143
VL - 30
SP - 1743
EP - 1750
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 8
ER -