Correlation between Bcl-X expression and B-Cell hybridoma apoptosis induced by activin A

Takeyoshi Koseki, Kenji Yamato, Akira Ishisaki, Osamu Hashimoto, Hiromu Sugino, Tatsuji Nishihara

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this study, we examined the role of bcl-XL and bcl-XS in apoptotic cell death of HS-72 cells induced by activin A. Immunoblot analysis revealed that a band of Bcl-XL was detected in HS-72 cells cultured with or without activin A. Although untreated HS-72 cells did not express Bcl-XS, the expression of Bcl-XS was significantly increased when cultured with activin A. We also investigated the expression of Bcl-XS and Bcl-XL in HS-72 cells cultured with activin A in the presence of protein kinase C inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H7), which suppressed apoptosis in HS-72 cells induced by activin A. Exposure to H7 apparently increased the level of Bcl-XL in HS-72 cells cultured with or without activin A. In contrast, no detectable hand of Bcl-XS was found in HS-72 cells cultured with activin A and H7. These findings indicate that Bcl-XL upregulation and Bcl-XS downregulation induced by H7 might correlate with the suppression of activin A-induced apoptosis in B-lineage cells.

Original languageEnglish
Pages (from-to)517-521
Number of pages5
JournalCellular Signalling
Volume10
Issue number7
DOIs
Publication statusPublished - 1998 Jul 1
Externally publishedYes

Keywords

  • Activin
  • Apoptosis
  • Bcl-2
  • Bcl-XL
  • Bcl-XS
  • Protein kinase

ASJC Scopus subject areas

  • Cell Biology

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