Correlation between angiogenesis and islet graft function in diabetic mice: Magnetic resonance imaging assessment

Naoaki Sakata, Andre Obenaus, Nathaniel K. Chan, Pete Hayes, John Chrisler, Eba Hathout

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Background/purpose: Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was used to evaluate neovascularization after intravenous injection of gadolinium, where contrast leaks out of new vessels and remains within the tissues. We examined the relationship between DCE-MRI and metabolic parameters such as blood glucose, serum insulin and glucose tolerance test (GTT) after intraportal islet transplantation. Methods: Streptozotocin-induced diabetic BALB/c mice (n = 15) received syngeneic intraportal islet transplantation (500 islet equivalent). Blood glucose, serum insulin and GTT were evaluated till postoperative day (POD) 14. Liver DCE-MRI was performed at POD 3, 7 and 14. Correlations between DCE-MRI and metabolic parameters were examined using regression analysis. Results: Eight mice achieved normoglycemia after intraportal transplantation. At POD 3 a significant but moderate correlation between DCE-MRI and blood glucose was found. No DCE-MRI or metabolic parameters correlated at POD 7. However, at POD 14 strong or moderate correlations between DCE-MRIs were found: negative correlations with blood glucose (R2 = 0.86) and GTT (R2 = 0.48) but a positive correlation with serum insulin (R2 = 0.32). Conclusion: We report that DCE-MRI can reflect the metabolic and functional condition of the transplanted islets.

Original languageEnglish
Pages (from-to)692-700
Number of pages9
JournalJournal of Hepato-Biliary-Pancreatic Sciences
Volume17
Issue number5
DOIs
Publication statusPublished - 2010 Sep

Keywords

  • Angiogenesis
  • Islet transplantation
  • Liver
  • Magnetic resonance imaging (MRI)

ASJC Scopus subject areas

  • Medicine(all)

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