Coronary vascular bed perfusion with a polyethylene glycol-modified hemoglobin-encapsulated liposome, Neo red cell, in rats

Kunihiko Nakai, Akira Usuba, Toshio Ohta, Mikinori Kuwabara, Yoshikazu Nakazato, Ryoichi Motoki, Tsuneo A. Takahashi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Whether hemoglobin (Hb) encapsulated liposomes have vasoconstrictive activity remains controversial. We therefore examined the vascular activity of a liposome Hb, Neo red cell (NRC), in a simple in vitro model of Langendorff perfusion of the rat heart using KrebsHenseleit (KH) solution as the perfusate. In the KH solution, NRC (Hb at 1 mg/ml), however, induced an immediate and abnormal increase in perfusion pressure. Histological examinations revealed that embolisms were the likely cause of this disturbance. Inorganic crystals formed by the mixing of NRC with the perfusate were a possible source of the embolisms. We found that the addition of bovine serum albumin to the perfusate was effective in avoiding embolic events. This protocol was used to compare the vasoconstrictive properties of unmodified bovine Hb and NRC. Unmodified bovine Hb (1 mg/ml) caused an increase in perfusion pressure and O decrease in the duration of bradykinin- induced relaxation. In contrast, NRC (Hb at 1 mg/ml) had no such vasoconstrictive effects. These results provide the first information regarding perfusion of the circulatory vascular bed by NRC and further evidence that the encapsulation of Hb into liposomes is an effective approach to modulate Hb-related vasoconstrictive activity.

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalArtificial Organs
Volume22
Issue number4
DOIs
Publication statusPublished - 1998

Keywords

  • Embolism
  • Hemoglobin
  • Langendorff perfusion
  • Liposomes
  • Neo red cell
  • Oxygen carrier
  • Vasoconstriction

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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