Core-shell beads made by composite liquid marble technology as a versatile microreactor for polymerase chain reaction

Kamalalayam Rajan Sreejith, Lena Gorgannezhad, Jing Jin, Chin Hong Ooi, Takayuki Takei, Gen Hayase, Helen Stratton, Krystina Lamb, Muhammad Shiddiky, Dzung Viet Dao, Nam Trung Nguyen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Over the last three decades, the protocols and procedures of the DNA amplification technique, polymerase chain reaction (PCR), have been optimized and well developed. However, there have been no significant innovations in processes for sample dispersion for PCR that have reduced the amount of single-use or unrecyclable plastic waste produced. To address the issue of plastic waste, this paper reports the synthesis and successful use of a core-shell bead microreactor using photopolymerization of a composite liquid marble as a dispersion process. This platform uses the core-shell bead as a simple and effective sample dispersion medium that significantly reduces plastic waste generated compared to conventional PCR processes. Other improvements over conventional PCR processes of the novel dispersion platform include increasing the throughput capability, enhancing the performance and portability of the thermal cycler, and allowing for the contamination-free storage of samples after thermal cycling.

Original languageEnglish
Article number242
JournalMicromachines
Volume11
Issue number3
DOIs
Publication statusPublished - 2020 Mar 1

Keywords

  • Composite liquid marble
  • Core-shell bead
  • Polymerase chain reaction (PCR)

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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    Sreejith, K. R., Gorgannezhad, L., Jin, J., Ooi, C. H., Takei, T., Hayase, G., Stratton, H., Lamb, K., Shiddiky, M., Dao, D. V., & Nguyen, N. T. (2020). Core-shell beads made by composite liquid marble technology as a versatile microreactor for polymerase chain reaction. Micromachines, 11(3), [242]. https://doi.org/10.3390/mi11030242