Copper-transporting P-type adenosine triphosphatase (ATP7B) is expressed in human breast carcinoma

Atsuko Kanzaki, Masakazu Toi, Nouri Neamati, Hitoshi Miyashita, Masahiro Oubu, Kentaro Nakayama, Hiroko Bando, Kenji Ogawa, Masato Mutoh, Shiro Mori, Kunihiko Terada, Toshihiro Sugiyama, Manabu Fukumoto, Yuji Takebayashi

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

This is the first report to show that a copper-transporting P-type adenosine triphosphatase, ATP7B, is expressed in certain breast carcinomas, and a priori knowledge of its expression is important for the choice of therapy. We investigated the hypothesis that ATP7B, which was shown to be associated with cisplatin resistance in vitro, is expressed in certain breast carcinomas. To test this hypothesis, ATP7B expression and protein level were examined in 41 breast carcinomas using RT-PCR and immunohistochemistry. ATP7B gene/protein could be detected in 22.0% (9/41) of breast carcinomas and ATP7B gene expression was correlated well with the protein expression. In nine ATP7B-positive tumors, adjacent normal breast tissue was similarly analyzed, revealing that ATP7B is upregulated in breast carcinoma. ATP7B gene expression in poorly differentiated carcinoma was significantly higher than that in well-/moderately differentiated carcinoma (P=0.012). Furthermore, we found no association between the ATP7B gene/protein expression and that of MDR1, MRP1, LRP and BCRP. These findings suggested that ATP7B gene expression might be a chemoresistance marker for cisplatin in patients with poorly differentiated breast carcinoma.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalJapanese Journal of Cancer Research
Volume93
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • ATP7B
  • Breast carcinoma
  • Cisplatin
  • MDR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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