Copper metallochaperones are required for the assembly of bacteroid cytochrome c oxidase which is functioning for nitrogen fixation in soybean nodules

Hatthaya Arunothayanan, Mika Nomura, Rie Hamaguchi, Manabu Itakura, Kiwamu Minamisawa, Shigeyuki Tajima

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Bradyrhizobium japonicum, a symbiotic nitrogen-fixing bacterium for Glycine max, has complex respiratory electron transport chains. Bll4880 contained a copper-binding motif for metallochaperone, H(M)X10MX21HXM. A mutant strain, Bj4880, induced nodules with lower acetylene reduction activity. A double mutant, Bj4880-1131, which had inserted mutations both in blr1131, a gene of the Sco1-like protein, and in bll4880, induced nodules of significant Fix- phenotype and low cytochrome c oxidase (Cco) activity in the bacteroid. Our data suggest that bll4880 protein is involved in copper ion delivery to Cco through blr1131 protein, and the expression of both proteins was induced under microaerobic conditions.

Original languageEnglish
Pages (from-to)1242-1246
Number of pages5
JournalPlant and Cell Physiology
Volume51
Issue number7
DOIs
Publication statusPublished - 2010 Jul

Keywords

  • Bradyrhizobium japonicum
  • Cytochrome c oxidase
  • Metallochaperone

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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