TY - JOUR
T1 - Copper metallochaperones are required for the assembly of bacteroid cytochrome c oxidase which is functioning for nitrogen fixation in soybean nodules
AU - Arunothayanan, Hatthaya
AU - Nomura, Mika
AU - Hamaguchi, Rie
AU - Itakura, Manabu
AU - Minamisawa, Kiwamu
AU - Tajima, Shigeyuki
N1 - Funding Information:
This work was supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan [Grant-in-Aid (19580067 to S.T) and the Special Coordination Funds for Promoting Science and Technology].
PY - 2010/7
Y1 - 2010/7
N2 - Bradyrhizobium japonicum, a symbiotic nitrogen-fixing bacterium for Glycine max, has complex respiratory electron transport chains. Bll4880 contained a copper-binding motif for metallochaperone, H(M)X10MX21HXM. A mutant strain, Bj4880, induced nodules with lower acetylene reduction activity. A double mutant, Bj4880-1131, which had inserted mutations both in blr1131, a gene of the Sco1-like protein, and in bll4880, induced nodules of significant Fix- phenotype and low cytochrome c oxidase (Cco) activity in the bacteroid. Our data suggest that bll4880 protein is involved in copper ion delivery to Cco through blr1131 protein, and the expression of both proteins was induced under microaerobic conditions.
AB - Bradyrhizobium japonicum, a symbiotic nitrogen-fixing bacterium for Glycine max, has complex respiratory electron transport chains. Bll4880 contained a copper-binding motif for metallochaperone, H(M)X10MX21HXM. A mutant strain, Bj4880, induced nodules with lower acetylene reduction activity. A double mutant, Bj4880-1131, which had inserted mutations both in blr1131, a gene of the Sco1-like protein, and in bll4880, induced nodules of significant Fix- phenotype and low cytochrome c oxidase (Cco) activity in the bacteroid. Our data suggest that bll4880 protein is involved in copper ion delivery to Cco through blr1131 protein, and the expression of both proteins was induced under microaerobic conditions.
KW - Bradyrhizobium japonicum
KW - Cytochrome c oxidase
KW - Metallochaperone
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U2 - 10.1093/pcp/pcq079
DO - 10.1093/pcp/pcq079
M3 - Article
C2 - 20519277
AN - SCOPUS:77954975365
VL - 51
SP - 1242
EP - 1246
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -