Copper metallochaperones are required for the assembly of bacteroid cytochrome c oxidase which is functioning for nitrogen fixation in soybean nodules

Hatthaya Arunothayanan, Mika Nomura, Rie Hamaguchi, Manabu Itakura, Kiwamu Minamisawa, Shigeyuki Tajima

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Bradyrhizobium japonicum, a symbiotic nitrogen-fixing bacterium for Glycine max, has complex respiratory electron transport chains. Bll4880 contained a copper-binding motif for metallochaperone, H(M)X10MX21HXM. A mutant strain, Bj4880, induced nodules with lower acetylene reduction activity. A double mutant, Bj4880-1131, which had inserted mutations both in blr1131, a gene of the Sco1-like protein, and in bll4880, induced nodules of significant Fix- phenotype and low cytochrome c oxidase (Cco) activity in the bacteroid. Our data suggest that bll4880 protein is involved in copper ion delivery to Cco through blr1131 protein, and the expression of both proteins was induced under microaerobic conditions.

    Original languageEnglish
    Pages (from-to)1242-1246
    Number of pages5
    JournalPlant and Cell Physiology
    Volume51
    Issue number7
    DOIs
    Publication statusPublished - 2010 Jul 1

    Keywords

    • Bradyrhizobium japonicum
    • Cytochrome c oxidase
    • Metallochaperone

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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