Copper electrodes for stable subpicomole detection of carbohydrates in high-performance liquid chromatography

Kenji Kano, Kazuyoshi Takagi, Kumi Inoue, Tokuji Ikeda, Teruhisa Ueda

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

A new pretreatment of copper (Cu) electrodes used for catalytic oxidation of carbohydrates is presented, which involves superficial polishing of the electrochemically-oxidized Cu electrode and then air-oxidation at 100°C for 3 h. This pretreatment allows highly sensitive and stable detection of carbohydrates at 0.45 V (vs. Ag/AgCl/Sat. KCl) in anion-exchange high-performance liquid chromatography. Glucose was detected at concentration levels as low as 0.2 pmole with a dynamic range up to 10,000 pmole over a period of 1 month. The change in the electrode surface state during the pretreatment is discussed from the electrochemical point of view.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalJournal of Chromatography A
Volume721
Issue number1
DOIs
Publication statusPublished - 1996 Jan 15
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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