Coordination of cellular dynamics contributes to tooth epithelium deformations

Ritsuko Morita, Miho Kihira, Yousuke Nakatsu, Yohei Nomoto, Miho Ogawa, Kazumasa Ohashi, Kensaku Mizuno, Tetsuhiko Tachikawa, Yukitaka Ishimoto, Yoshihiro Morishita, Takashi Tsuji

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The morphologies of ectodermal organs are shaped by appropriate combinations of several deformation modes, such as invagination and anisotropic tissue elongation. However, how multicellular dynamics are coordinated during deformation processes remains to be elucidated. Here, we developed a four-dimensional (4D) analysis system for tracking cell movement and division at a single-cell resolution in developing tooth epithelium. The expression patterns of a Fucci probe clarified the region- and stage-specific cell cycle patterns within the tooth germ, which were in good agreement with the pattern of the volume growth rate estimated from tissue-level deformation analysis. Cellular motility was higher in the regions with higher growth rates, while the mitotic orientation was significantly biased along the direction of tissue elongation in the epithelium. Further, these spatio-temporal patterns of cellular dynamics and tissue-level deformation were highly correlated with that of the activity of cofilin, which is an actin depolymerization factor, suggesting that the coordination of cellular dynamics via actin remodeling plays an important role in tooth epithelial morphogenesis. Our system enhances the understanding of how cellular behaviors are coordinated during ectodermal organogenesis, which cannot be observed from histological analyses.

    Original languageEnglish
    Article numbere0161336
    JournalPloS one
    Volume11
    Issue number9
    DOIs
    Publication statusPublished - 2016 Sep

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

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    Morita, R., Kihira, M., Nakatsu, Y., Nomoto, Y., Ogawa, M., Ohashi, K., Mizuno, K., Tachikawa, T., Ishimoto, Y., Morishita, Y., & Tsuji, T. (2016). Coordination of cellular dynamics contributes to tooth epithelium deformations. PloS one, 11(9), [e0161336]. https://doi.org/10.1371/journal.pone.0161336