Coordinated transcriptional regulation of isopentenyl diphosphate biosynthetic pathway enzymes in plastids by phytochrome-interacting factor 5

Kazuto Mannen, Takuro Matsumoto, Seiji Takahashi, Yuta Yamaguchi, Masanori Tsukagoshi, Ryosuke Sano, Hideyuki Suzuki, Nozomu Sakurai, Daisuke Shibata, Tanetoshi Koyama, Toru Nakayama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

All isoprenoids are derived from a common C5 unit, isopentenyl diphosphate (IPP). In plants, IPP is synthesized via two distinct pathways; the cytosolic mevalonate pathway and the plastidial non-mevalonate (MEP) pathway. In this study, we used a co-expression analysis to identify transcription factors that coordinately regulate the expression of multiple genes encoding enzymes in the IPP biosynthetic pathway. Some candidates showed especially strong correlations with multiple genes encoding MEP-pathway enzymes. We report here that phytochrome-interacting factor 5 (PIF5), a basic-helix-loop-helix type transcription factor, functions as a positive regulator of the MEP pathway. Its overexpression in T87 suspension cultured cells resulted in increased accumulation of chlorophylls and carotenoids. Detailed analyses of carotenoids by HPLC indicated that some carotenoid biosynthetic pathways were concomitantly up-regulated, possibly as a result of enhanced IPP metabolic flow. Our results also revealed other PIF family proteins that play different roles from that of PIF5 in IPP metabolism.

Original languageEnglish
Pages (from-to)768-774
Number of pages7
JournalBiochemical and biophysical research communications
Volume443
Issue number2
DOIs
Publication statusPublished - 2014 Jan 10

Keywords

  • Arabidopsis thaliana
  • Co-expression analysis
  • Isoprenoids
  • Phytochrome-interacting factor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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