Cooperative DNA probing using a β-cyclodextrin DNA conjugate and a nucleobase-specific fluorescent ligand

Toshihiro Ihara, Asuka Uemura, Akika Futamura, Masamichi Shimizu, Noriyuki Baba, Seiichi Nishizawa, Norio Teramae, Akinori Jyo

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

A single nucleotide polymorphism (SNP) base on the target is displayed at a gap in a ternary duplex carrying A-cyclodextrin-modified DNA. A stable tandem duplex forms regardless of the type of SNP base. A nucleobase-specific ligand is then added to this system. The dansyl moiety in the ligand is expected to form a luminous inclusion complex with nearby A-CyD, only when the ligand recognizes the specific base displayed in the gap.

Original languageEnglish
Pages (from-to)1386-1387
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number4
DOIs
Publication statusPublished - 2009 Feb 4

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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