Cooperation between mDia1 and ROCK in Rho-induced actin reorganization

Naoki Watanabe, Takayuki Kato, Akiko Fujita, Toshimasa Ishizaki, Shuh Narumiya

    Research output: Contribution to journalArticlepeer-review

    687 Citations (Scopus)

    Abstract

    The small GTPase Rho induces the formation of actin stress fibres and mediates the formation of diverse actin structures. However, it remains unclear how Rho regulates its effectors to elicit such functions. Here we show that GTP-bound Rho activates its effector mDia1 by disrupting mDia1's intramolecular interactions. Active mDia1 induces the formation of thin actin stress fibres, which are disorganized in the absence of activity of the Rho-associated kinase ROCK. Moreover, active mDia1 transforms ROCK-induced condensed actin fibres into structures reminiscent of Rho-induced stress fibres. Thus mDia1 and ROCK work concurrently during Rho-induced stress-fibre formation. Intriguingly, mDia1 and ROCK, depending on the balance of the two activities, induce actin fibres of various thicknesses and densities. Thus Rho may induce the formation of different actin structures affected by the balance between mDia1 and ROCK signalling.

    Original languageEnglish
    Pages (from-to)136-143
    Number of pages8
    JournalNature cell biology
    Volume1
    Issue number3
    DOIs
    Publication statusPublished - 1999 Jul

    ASJC Scopus subject areas

    • Cell Biology

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