Cooling treatment transiently increases the permeability of brain capillary endothelial cells through translocation of claudin-5

Akinori Inamura, Yasuhiro Adachi, Takao Inoue, Yeting He, Nobuko Tokuda, Takashi Nawata, Satoshi Shirao, Sadahiro Nomura, Masami Fujii, Eiji Ikeda, Yuji Owada, Michiyasu Suzuki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The blood-brain-barrier (BBB) is formed by different cell types, of which brain microvascular endothelial cells are major structural constituents. The goal of this study was to examine the effects of cooling on the permeability of the BBB with reference to tight junction formation of brain microendothelial cells. The sensorimotor cortex above the dura mater in adult male Wistar rats was focally cooled to a temperature of 5 C for 1 h, then immunostaining for immunoglobulin G (IgG) was performed to evaluate the permeability of the BBB. Permeability produced by cooling was also evaluated in cultured murine brain endothelial cells (bEnd3) based on measurement of trans-epithelial electric resistance (TEER). Immunocytochemistry and Western blotting of proteins associated with tight junctions in bEnd3 were performed to determine protein distribution before and after cooling. After focal cooling of the rat brain cortex, diffuse immunostaining for IgG was observed primarily around the small vasculature and in the extracellular spaces of parenchyma of the cortex. In cultured bEnd3, TEER significantly decreased during cooling (15 C) and recovered to normal levels after rewarming to 37 C. Immunocytochemistry and Western blotting showed that claudin-5, a critical regulatory protein for tight junctions, was translocated from the membrane to the cytoplasm after cooling in cultured bEnd3 cells. These results suggest that focal brain cooling may open the BBB transiently through an effect on tight junctions of brain microendothelial cells, and that therapeutically this approach may allow control of BBB function and drug delivery through the BBB.

Original languageEnglish
Pages (from-to)1641-1647
Number of pages7
JournalNeurochemical Research
Volume38
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1
Externally publishedYes

Keywords

  • Claudin-5
  • Endothelial cells
  • Focal brain cooling
  • TEER (trans-epithelial electric resistance)
  • Tight junction

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Inamura, A., Adachi, Y., Inoue, T., He, Y., Tokuda, N., Nawata, T., Shirao, S., Nomura, S., Fujii, M., Ikeda, E., Owada, Y., & Suzuki, M. (2013). Cooling treatment transiently increases the permeability of brain capillary endothelial cells through translocation of claudin-5. Neurochemical Research, 38(8), 1641-1647. https://doi.org/10.1007/s11064-013-1066-4