Converging circuits mediate temperature and shock aversive olfactory conditioning in Drosophila

Dana Shani Galili, Kristina V. Dylla, Alja Lüdke, Anja B. Friedrich, Nobuhiro Yamagata, Jin Yan Hilary Wong, Chien Hsien Ho, Paul Szyszka, Hiromu Tanimoto

    Research output: Contribution to journalArticlepeer-review

    43 Citations (Scopus)

    Abstract

    Background Drosophila learn to avoid odors that are paired with aversive stimuli. Electric shock is a potent aversive stimulus that acts via dopamine neurons to elicit avoidance of the associated odor. While dopamine signaling has been demonstrated to mediate olfactory electric shock conditioning, it remains unclear how this pathway is involved in other types of behavioral reinforcement, such as in learned avoidance of odors paired with increased temperature. Results To better understand the neural mechanisms of distinct aversive reinforcement signals, we here established an olfactory temperature conditioning assay comparable to olfactory electric shock conditioning. We show that the AC neurons, which are internal thermal receptors expressing dTrpA1, are selectively required for odor-temperature but not for odor-shock memory. Furthermore, these separate sensory pathways for increased temperature and shock converge onto overlapping populations of dopamine neurons that signal aversive reinforcement. Temperature conditioning appears to require a subset of the dopamine neurons required for electric shock conditioning. Conclusions We conclude that dopamine neurons integrate different noxious signals into a general aversive reinforcement pathway.

    Original languageEnglish
    Pages (from-to)1712-1722
    Number of pages11
    JournalCurrent Biology
    Volume24
    Issue number15
    DOIs
    Publication statusPublished - 2014 Aug 4

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Fingerprint

    Dive into the research topics of 'Converging circuits mediate temperature and shock aversive olfactory conditioning in Drosophila'. Together they form a unique fingerprint.

    Cite this