Controlling the folding/unfolding transition of the DNA-histone H1 complex by direct optical manipulation

Yuko Yoshikawa, Shin Ichirou M. Nomura, Toshio Kanbe, Kenichi Yoshikawa

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Single giant duplex DNA (T4DNA, 166 kbp) complexed with a polycationic protein, histone H1, is trapped by a continuous-wave infrared laser, without chemical modification of the DNA such as attachment to a micrometer-sized bead. When the DNA-H1 complex is transported from a low-salt solution (0.2 M NaCl) to a high-salt solution (2 M NaCl), its conformation changes from a compact to an elongated structure. With the reverse procedure, i.e., with the transportation from the high-salt to the low-salt environment, the elongated chain shrinks into a compact state. These results afford the direct evidence that histone H1 induces a salt-dependent transition in the higher-order structure of giant DNA molecules.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalChemical Physics Letters
Volume330
Issue number1-2
DOIs
Publication statusPublished - 2000 Nov 3
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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