TY - JOUR
T1 - Controlled conversion of sodium hyaluronate into low-molecular-weight polymers without additives using high-temperature water and fast-heating-rates
AU - Aida, Taku
AU - Oshima, Minori
AU - Sharmin, Tanjina
AU - Mishima, Kenji
AU - Smith, Richard L.
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Number JP15K06535 and Fukuoka University Research Institute of Composite Materials. We would like to thank Dr. Masato Noguchi (Tohoku University Japan) for 1H-NMR analysis.
Funding Information:
This work was supported by JSPS KAKENHI Grant Number JP15K06535 and Fukuoka University Research Institute of Composite Materials . We would like to thank Dr. Masato Noguchi (Tohoku University Japan) for 1 H-NMR analysis. Appendix A
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/1
Y1 - 2020/1
N2 - In this work, molecular weight reduction of the polysaccharide biopolymer, sodium hyaluronate (NaHA), was obtained without added chemicals or catalysts using high temperature water (180 to 260) °C, fast-heating-rates (417 to 750) °C∙s−1 and short reaction times (< 18 s) in a flow reactor. Results show that hydrothermal treatment of NaHA with fast-heating-rates convert the substrate into low molecular weight oligomers with minimal byproduct formation. A kinetic model assuming random depolymerization provided data correlation and allowed estimation of molecular weight reductions. Mechanism of NaHA decomposition in high temperature water with fast-heating rates seems to be related to initial disentanglement of NaHA random coils through convective (turbulent) mixing and shear that minimizes heterogenous decomposition. Evidence for the mechanism includes lack of significant furan byproducts (fast-heating), clear product solutions and model applicability. Fast-heating with high temperature water allows selective conversion of high molecular weight NaHA into low molecular weight HA fragments without chemical modification, catalysts or additives.
AB - In this work, molecular weight reduction of the polysaccharide biopolymer, sodium hyaluronate (NaHA), was obtained without added chemicals or catalysts using high temperature water (180 to 260) °C, fast-heating-rates (417 to 750) °C∙s−1 and short reaction times (< 18 s) in a flow reactor. Results show that hydrothermal treatment of NaHA with fast-heating-rates convert the substrate into low molecular weight oligomers with minimal byproduct formation. A kinetic model assuming random depolymerization provided data correlation and allowed estimation of molecular weight reductions. Mechanism of NaHA decomposition in high temperature water with fast-heating rates seems to be related to initial disentanglement of NaHA random coils through convective (turbulent) mixing and shear that minimizes heterogenous decomposition. Evidence for the mechanism includes lack of significant furan byproducts (fast-heating), clear product solutions and model applicability. Fast-heating with high temperature water allows selective conversion of high molecular weight NaHA into low molecular weight HA fragments without chemical modification, catalysts or additives.
KW - Drug delivery
KW - Extracellular-matrix
KW - Hyaluronic acid
KW - Hydrothermal
KW - Reaction kinetics
KW - Reaction mechanism
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U2 - 10.1016/j.supflu.2019.104638
DO - 10.1016/j.supflu.2019.104638
M3 - Article
AN - SCOPUS:85072924325
VL - 155
JO - Journal of Supercritical Fluids
JF - Journal of Supercritical Fluids
SN - 0896-8446
M1 - 104638
ER -