Control of strain in subgrains of protein crystals by the introduction of grown-in dislocations

Haruhiko Koizumi, Satoshi Uda, Ryo Suzuki, Masaru Tachibana, Kenichi Kojima, Katsuo Tsukamoto, Izumi Yoshizaki, Seijiro Fukuyama, Yoshihisa Suzuki

Research output: Contribution to journalArticlepeer-review

Abstract

It is important to reveal the exact cause of poor diffractivity in protein crystals in order to determine the accurate structure of protein molecules. It is shown that there is a large amount of local strain in subgrains of glucose isomerase crystals even though the overall crystal quality is rather high, as shown by clear equal-Thickness fringes in X-ray topography. Thus, a large stress is exerted on the subgrains of protein crystals, which could significantly lower the resistance of the crystals to radiation damage. It is also demonstrated that this local strain can be reduced through the introduction of dislocations in the crystal. This suggests that the introduction of dislocations in protein crystals can be effective in enhancing the crystal quality of subgrains of protein crystals. By exploiting this effect, the radiation damage in subgrains could be decreased, leading to the collection of X-ray diffraction data sets with high diffractivity.

Original languageEnglish
Pages (from-to)599-605
Number of pages7
JournalActa Crystallographica Section D: Structural Biology
Volume77
DOIs
Publication statusPublished - 2021 May 1

Keywords

  • crystal quality
  • local strain
  • protein crystals
  • rocking curves.
  • subgrains

ASJC Scopus subject areas

  • Structural Biology

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