Control of neural stem cell differentiation on honeycomb films

Akinori Tsuruma, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura

    Research output: Contribution to journalArticlepeer-review

    54 Citations (Scopus)


    Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 μm pore specifically.

    Original languageEnglish
    Pages (from-to)536-540
    Number of pages5
    JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
    Publication statusPublished - 2008 Feb 1


    • Cell differentiation
    • Micro and nano-pattern
    • Neural regeneration
    • Neural stem cell
    • Neuron

    ASJC Scopus subject areas

    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry


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