Control of K+ conductance by cholecystokinin and Ca2+ in single pancreatic acinar cells studied by the patch-clamp technique

Yoshio Maruyama, Ole H. Petersen

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The effect of cholecystokinin (CCK) and internal Ca2+ on outward K+ current in isolated pig pancreatic acinar cells has been investigated using the patch-clamp method for whole-cell current recording under voltage-clamp conditions. CCK (2 × 10-10M) applied to the bath evoked a marked increase in the outward K+ current associated with depolarizing voltage steps, and this effect was fully reversible and acutely dependent on the presence of external Ca2+. When strongly buffered Ca2+-EGTA solutions were used inside the cells CCK failed to evoke an effect. Increasing the internal Ca2+ concentration ([Ca2+]i) from 5 × 10-10M to 10-7 and 5 × 10-7M mimicked the effect of CCK. It would appear therefore that CCK controls K+ conductance in the acinar cells via changes in the internal free ionized Ca2+ concentration.

Original languageEnglish
Pages (from-to)293-298
Number of pages6
JournalThe Journal of Membrane Biology
Volume79
Issue number3
DOIs
Publication statusPublished - 1984 Oct 1

Keywords

  • Ca control
  • K channel
  • cholecystokinin
  • pancreatic acinar cells
  • patch clamp
  • single channel recording
  • whole cell recording

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Control of K<sup>+</sup> conductance by cholecystokinin and Ca<sup>2+</sup> in single pancreatic acinar cells studied by the patch-clamp technique'. Together they form a unique fingerprint.

Cite this