Control of K+ conductance by cholecystokinin and Ca2+ in single pancreatic acinar cells studied by the patch-clamp technique

Yoshio Maruyama; Ole H. Petersen

doi:10.1007/BF01871068

Control of K⁺ conductance by cholecystokinin and Ca²⁺ in single pancreatic acinar cells studied by the patch-clamp technique

Yoshio Maruyama, Ole H. Petersen

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The effect of cholecystokinin (CCK) and internal Ca²⁺ on outward K⁺ current in isolated pig pancreatic acinar cells has been investigated using the patch-clamp method for whole-cell current recording under voltage-clamp conditions. CCK (2 × 10^-10M) applied to the bath evoked a marked increase in the outward K⁺ current associated with depolarizing voltage steps, and this effect was fully reversible and acutely dependent on the presence of external Ca²⁺. When strongly buffered Ca²⁺-EGTA solutions were used inside the cells CCK failed to evoke an effect. Increasing the internal Ca²⁺ concentration ([Ca²⁺]_i) from 5 × 10^-10M to 10^-7 and 5 × 10^-7M mimicked the effect of CCK. It would appear therefore that CCK controls K⁺ conductance in the acinar cells via changes in the internal free ionized Ca²⁺ concentration.

Original language	English
Pages (from-to)	293-298
Number of pages	6
Journal	The Journal of Membrane Biology
Volume	79
Issue number	3
DOIs	https://doi.org/10.1007/BF01871068
Publication status	Published - 1984 Oct 1

Keywords

Ca control
K channel
cholecystokinin
pancreatic acinar cells
patch clamp
single channel recording
whole cell recording

ASJC Scopus subject areas

Biophysics
Physiology
Cell Biology

Access to Document

10.1007/BF01871068

Cite this

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title = "Control of K+ conductance by cholecystokinin and Ca2+ in single pancreatic acinar cells studied by the patch-clamp technique",

abstract = "The effect of cholecystokinin (CCK) and internal Ca2+ on outward K+ current in isolated pig pancreatic acinar cells has been investigated using the patch-clamp method for whole-cell current recording under voltage-clamp conditions. CCK (2 × 10-10M) applied to the bath evoked a marked increase in the outward K+ current associated with depolarizing voltage steps, and this effect was fully reversible and acutely dependent on the presence of external Ca2+. When strongly buffered Ca2+-EGTA solutions were used inside the cells CCK failed to evoke an effect. Increasing the internal Ca2+ concentration ([Ca2+]i) from 5 × 10-10M to 10-7 and 5 × 10-7M mimicked the effect of CCK. It would appear therefore that CCK controls K+ conductance in the acinar cells via changes in the internal free ionized Ca2+ concentration.",

keywords = "Ca control, K channel, cholecystokinin, pancreatic acinar cells, patch clamp, single channel recording, whole cell recording",

author = "Yoshio Maruyama and Petersen, {Ole H.}",

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T1 - Control of K+ conductance by cholecystokinin and Ca2+ in single pancreatic acinar cells studied by the patch-clamp technique

AU - Maruyama, Yoshio

AU - Petersen, Ole H.

PY - 1984/10/1

Y1 - 1984/10/1

N2 - The effect of cholecystokinin (CCK) and internal Ca2+ on outward K+ current in isolated pig pancreatic acinar cells has been investigated using the patch-clamp method for whole-cell current recording under voltage-clamp conditions. CCK (2 × 10-10M) applied to the bath evoked a marked increase in the outward K+ current associated with depolarizing voltage steps, and this effect was fully reversible and acutely dependent on the presence of external Ca2+. When strongly buffered Ca2+-EGTA solutions were used inside the cells CCK failed to evoke an effect. Increasing the internal Ca2+ concentration ([Ca2+]i) from 5 × 10-10M to 10-7 and 5 × 10-7M mimicked the effect of CCK. It would appear therefore that CCK controls K+ conductance in the acinar cells via changes in the internal free ionized Ca2+ concentration.

AB - The effect of cholecystokinin (CCK) and internal Ca2+ on outward K+ current in isolated pig pancreatic acinar cells has been investigated using the patch-clamp method for whole-cell current recording under voltage-clamp conditions. CCK (2 × 10-10M) applied to the bath evoked a marked increase in the outward K+ current associated with depolarizing voltage steps, and this effect was fully reversible and acutely dependent on the presence of external Ca2+. When strongly buffered Ca2+-EGTA solutions were used inside the cells CCK failed to evoke an effect. Increasing the internal Ca2+ concentration ([Ca2+]i) from 5 × 10-10M to 10-7 and 5 × 10-7M mimicked the effect of CCK. It would appear therefore that CCK controls K+ conductance in the acinar cells via changes in the internal free ionized Ca2+ concentration.

KW - Ca control

KW - K channel

KW - cholecystokinin

KW - pancreatic acinar cells

KW - patch clamp

KW - single channel recording

KW - whole cell recording

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