Abstract
The effect of cholecystokinin (CCK) and internal Ca2+ on outward K+ current in isolated pig pancreatic acinar cells has been investigated using the patch-clamp method for whole-cell current recording under voltage-clamp conditions. CCK (2 × 10-10M) applied to the bath evoked a marked increase in the outward K+ current associated with depolarizing voltage steps, and this effect was fully reversible and acutely dependent on the presence of external Ca2+. When strongly buffered Ca2+-EGTA solutions were used inside the cells CCK failed to evoke an effect. Increasing the internal Ca2+ concentration ([Ca2+]i) from 5 × 10-10M to 10-7 and 5 × 10-7M mimicked the effect of CCK. It would appear therefore that CCK controls K+ conductance in the acinar cells via changes in the internal free ionized Ca2+ concentration.
Original language | English |
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Pages (from-to) | 293-298 |
Number of pages | 6 |
Journal | The Journal of Membrane Biology |
Volume | 79 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1984 Oct 1 |
Keywords
- Ca control
- K channel
- cholecystokinin
- pancreatic acinar cells
- patch clamp
- single channel recording
- whole cell recording
ASJC Scopus subject areas
- Biophysics
- Physiology
- Cell Biology