Control of hepatocyte adhesion and function on self-organized honeycomb-patterned polymer film

Masaru Tanaka, Kazutaka Nishikawa, Hisashi Okubo, Hirofumi Kamachi, Tomoaki Kawai, Michiaki Matsushita, Satoru Todo, Masatsugu Shimomura

    Research output: Contribution to journalArticlepeer-review

    71 Citations (Scopus)


    Hepatocytes were cultured on a honeycomb-patterned polymer film (honeycomb film) formed by self-organization in order to investigate the influence of the honeycomb pattern on cell behavior. The changes in cell morphologies and actin filaments were observed by optical, fluorescence, and scanning electron microscopy. Hepatocytes were flattened, and the actin filaments appeared conspicuously in the spreading regions on a flat film. In contrast, the hepatocytes that were cultured on the honeycomb film were observed to form a spherical shape, and the actin filaments were localized inside the edge of the spheroid. The spheroids were observed within several hours after seeding on the honeycomb film; they were attached and the spheroid shape was maintained without any deformation. The spheroids expressed a higher level of liver specific function than the cell monolayers on the flat film. These results suggest that the honeycomb film is a suitable material for tissue engineering scaffolds and biomedical devices.

    Original languageEnglish
    Pages (from-to)464-469
    Number of pages6
    JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
    Publication statusPublished - 2006 Aug 15


    • Cell adhesion
    • Cell surface interaction
    • Hepatic function
    • Hepatocyte
    • Self-organization

    ASJC Scopus subject areas

    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry


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