Control of cellular adhesion within microfluidic device using electrochemical biolithography and dielectrophoresis for cell-based assay

M. Hashimoto, H. Kaji, M. Nishizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe a method to selectively immobilize a type of leucocytes (neutrophils or eosinophils) from a suspension of mixed leucocytes on the site-specific region inside a microchannel. One of the microelectrodes fabricated at the upper wall of the channel was used for locally electrogenerating hypobromous acid that renders the opposite face of the channel protein-adsorptive. Since the patterned layer of poly(ethylene glycol)dimethacrylate, impervious to HBrO, is previously introduced at the channel bottom, the region of protein adsorption can be precisely defined even in the presence of fluid-flow. After antibody was immobilized on the treated region, an ac voltage (1 MHz, 20 Vpp) was applied to the microelectrodes array in the presence of cell suspension flowing through the channel in order to concentrate cells onto the antibody-immobilized region by negative dielectrophoresis force. The results show that the immobilized antibody would selectively recognize the corresponding leucocyte type from the trapped cells to allow their adhesion.

Original languageEnglish
Title of host publicationECS Transactions - Microfabricated and Nanofabricated Systems for MEMS/NEMS 8
Pages11-20
Number of pages10
Edition14
DOIs
Publication statusPublished - 2008 Dec 1
EventMicrofabricated and Nanofabricated Systems for MEMS/NEMS 8 PRiMe 2008 Meeting of the Electrochemical Society - Honolulu, HI, United States
Duration: 2008 Oct 122008 Oct 17

Publication series

NameECS Transactions
Number14
Volume16
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Other

OtherMicrofabricated and Nanofabricated Systems for MEMS/NEMS 8 PRiMe 2008 Meeting of the Electrochemical Society
CountryUnited States
CityHonolulu, HI
Period08/10/1208/10/17

ASJC Scopus subject areas

  • Engineering(all)

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    Hashimoto, M., Kaji, H., & Nishizawa, M. (2008). Control of cellular adhesion within microfluidic device using electrochemical biolithography and dielectrophoresis for cell-based assay. In ECS Transactions - Microfabricated and Nanofabricated Systems for MEMS/NEMS 8 (14 ed., pp. 11-20). (ECS Transactions; Vol. 16, No. 14). https://doi.org/10.1149/1.2992223