Contribution of IRF-3 mediated IFNβ production to DNA vaccine dependent cellular immune responses

Hidekazu Shirota, Lev Petrenko, Toshio Hattori, Dennis M. Klinman

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


The mechanism(s) by which DNA vaccines activate Ag-specific cellular immune responses is incompletely understood. Current findings indicate that IRF-3 plays an important role in this process. The IRF-3 dependent signaling pathway is triggered by the presence of intracytoplasmic DNA, and culminates in the production of type I IFNs. DNA vaccination of IRF-3 KO mice elicits a strong Ag-specific humoral response, yet CD4 and CD8 T cell responses (including the production of Th1, Th2 and Th17 cytokines) are severely impaired. Although expression of the immunogenic protein encoded by the DNA vaccine was similar in IRF-3 KO vs wild type mice, antigen presentation was severely impaired in the KO animals. This defect was remedied by the co-delivery of an IFNβ encoding plasmid. These findings suggest that the IRF-3/IFNβ pathways are key to the induction of cellular immunity following DNA vaccination.

Original languageEnglish
Pages (from-to)2144-2149
Number of pages6
Issue number15
Publication statusPublished - 2009 Mar 26


  • DNA vaccine
  • IRF-3
  • Type1 IFNs

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases


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