Contrasting responses of human gingival and colonic epithelial cells to lipopolysaccharides, lipoteichoic acids and peptidoglycans in the presence of soluble cd14

Akiko Uehara, Shunji Sugawara, Riyoko Tamai, Haruhiko Takada

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Gingival epithelial cells may form the first barriers of defense against oral bacteria in periodontal tissues. We stimulated human gingival epithelial cells (keratinocytes) in primary culture, the oral epithelial cell line KB and the colonic epithelial cell line SW620 with various bacterial cell-surface components in the presence or absence of soluble CD14 (sCD14). The SW620 produced interlukin-8 (IL-8) in an sCD14-dependent manner in response to lipopolysaccharide, lipoteichoic acid and peptidoglycan. However, the primary gingival epithelial cells and KB cells did not show enhanced production of IL-8 upon stimulation with these components even in the presence of serum. These human epithelial cells were devoid of membrane CD14, as determined by flow cytometry, and CD14 mRNA expression, as determined by reverse transcriptase-PCR. In contrast, gingival epithelial cells and KB cells expressed the mRNA expression for Toll-like receptor (TLR) 2, TLR4, MD2 and MyD88 to the similar extent to those observed in SW620 cells.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalMedical Microbiology and Immunology
Volume189
Issue number4
DOIs
Publication statusPublished - 2001 Sep 22

Keywords

  • Gingival epithelial cells
  • Lipopolysaccharide
  • Lipoteichoic acids
  • Peptidoglycans
  • Soluble CD14

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Contrasting responses of human gingival and colonic epithelial cells to lipopolysaccharides, lipoteichoic acids and peptidoglycans in the presence of soluble cd14'. Together they form a unique fingerprint.

Cite this