Contra-IL 2; A suppressor lymphokine that inhibits IL 2 activity

T. Maki, S. Satomi, M. Gotoh, A. P. Monaco

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Suppressive activity of culture supernatants of AS-9 (AS-9 CS), a T cell hybridoma line that was derived from fusion of BW5147 thymoma and splenic T cells of anti-lymphocyte serum-treated C3H mice, was analyzed. AS-9 CS inhibited allogeneic cytotoxic T lymphocyte (CTL) generation as well as T cell proliferation to alloantigens and mitogens, but failed to inhibit B cell response to lipopolysaccharide or growth of tumor and fibroblast cells. Although addition of AS-9 CS to the allogeneic sensitization culture as late as on day 2 of incubation resulted in maximal inhibition of CTL generation, removal of AS-9-CS on day 3 of incubation abolished its inhibitory effect. Addition of purified IL 2 together with AS-9 CS to the allogeneic sensitization cultures only partially abrogated the suppression. Experiments with IL 2-dependent cytotoxic T cell line (CTLL) showed that AS-9 CS suppressed the IL 2-induced proliferation of CTLL. Preincubation of AS-9 CS with CTLL removed its inhibitory effect on CTL generation. These results indicate that AS-9 CS interferes with the mechanism of T cell activation by IL 2. On this basis, AS-9 CS was named contra-IL 2.

Original languageEnglish
Pages (from-to)3298-3304
Number of pages7
JournalJournal of Immunology
Volume136
Issue number9
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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