Continuous separation of proteins by recycling free-flow isoelectric focusing with crude feed supply and purified product removal

Toshikuni Yonemoto, Wataru Shirai, Teiriki Tadaki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A novel electrophoretic device with continuous crude feed supply and purified product removal ports was developed by modification of a batch type recycling free-flow isoelectric focusing device. The effectiveness of the device was verified by separation experiments in which bovine serum albumin and bovine hemoglobin were used as model ampholytes. High resolving power and continuous loading ability were attained with the device. The effect of concentration of the supporting electrolyte on the separation efficiency was also studied. Higher supporting electrolyte concentration in the initial loaded solution and lower concentration in the continuous feed solution were suitable for the efficient separation of the two ampholytes.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalBioseparation
Volume5
Issue number5
Publication statusPublished - 1995 Oct 1

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Chemical Engineering(all)

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