Continuous release of O2/ONOO in plasma-exposed HEPES-buffered saline promotes TRP channel-mediated uptake of a large cation

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Abstract

Although the externally controllable extracellular supply of the short-lived reactive oxygen and nitrogen species, such as O2•−, NO, and ONOO, could potentially manipulate cellular functions, their simple administration to cells is likely to be ineffective due to their rapid deactivation. In this study, we found a method of a continuous supply of O2•−/ONOO over a few minutes, which is triggered by irradiation of a nonequilibrium atmospheric pressure plasma to commonly used organic buffers (e.g., 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, HEPES). In addition, a continuous low-dose O2•−/ONOO supply was shown to induce a physiologically relevant Ca2+ response and subsequently the uptake of a large cation mediated by transient receptor potential channel family member(s). Our results provide a novel approach to the continuous O2•−/ONOO supply, requiring controllable and mass-volume treatments.

Original languageEnglish
Article number1900257
JournalPlasma Processes and Polymers
Volume17
Issue number10
DOIs
Publication statusPublished - 2020 Oct 1

Keywords

  • Ca signaling
  • TRP channel
  • atmospheric pressure plasma
  • peroxynitrite
  • plasma medicine

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Polymers and Plastics

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