Continuous release of O2 /ONOO in plasma-exposed HEPES-buffered saline promotes TRP channel-mediated uptake of a large cation

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Abstract

Although the externally controllable extracellular supply of the short-lived reactive oxygen and nitrogen species, such as O2 •−, NO, and ONOO, could potentially manipulate cellular functions, their simple administration to cells is likely to be ineffective due to their rapid deactivation. In this study, we found a method of a continuous supply of O2 •−/ONOO over a few minutes, which is triggered by irradiation of a nonequilibrium atmospheric pressure plasma to commonly used organic buffers (e.g., 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, HEPES). In addition, a continuous low-dose O2 •−/ONOO supply was shown to induce a physiologically relevant Ca2+ response and subsequently the uptake of a large cation mediated by transient receptor potential channel family member(s). Our results provide a novel approach to the continuous O2 •−/ONOO supply, requiring controllable and mass-volume treatments.

Original languageEnglish
JournalPlasma Processes and Polymers
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • Ca signaling
  • TRP channel
  • atmospheric pressure plasma
  • peroxynitrite
  • plasma medicine

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Polymers and Plastics

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