Continuous Measurement of Glutamate and Hydrogen Peroxide Using a Microfabricated Biosensor for Studying the Neurotoxicity of Tributyltin

Ryoji Kurita, Katsuyoshi Hayashi, Keiichi Torimitsu, Osamu Niwa

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We first measured the effects of trace levels of an endocrine disruptor, tributyltin (TBT), on the secretion response from nerve cells using a microfabricated biosensor designed for the continuous measurement of L-glutamate and hydrogen peroxide. We observed higher and long-lasting glutamate and hydrogen peroxide concentrations from the cells when cultured rat cortical neurons were exposed to TBT. Glutamate and hydrogen peroxide release was induced even when we reduced the TBT concentration to 10 nM. This concentration is about two orders of magnitude lower than the concentration that induced apoptosis-like cell death. We also report on the effects of NMDA and non-NMDA receptor antagonists, which can help us to understand the mechanism of TBT neurotoxicity.

Original languageEnglish
Pages (from-to)1581-1585
Number of pages5
Journalanalytical sciences
Volume19
Issue number12
DOIs
Publication statusPublished - 2003 Dec

ASJC Scopus subject areas

  • Analytical Chemistry

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