Continuous collection and simultaneous detection of picoliter volume of nucleic acid samples using a mille-feuille probe

Hidenori Ito, Motoki Tanaka, Yuanshu Zhou, Yuji Nashimoto, Yasufumi Takahashi, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Investigation of the positional heterogeneity of messenger RNA (mRNA) expression in tissues requires a technology that facilitates analysis of mRNA expression in the selected single cells. We developed a mille-feuille probe (MP) that allows the lamination of the aqueous and organic phases in a nanopipette under voltage control. The MP was used for continuous collection of different nucleic acid samples and sequential evaluation of gene expression with mRNA barcoding tags. First, we found that the aqueous phases could be laminated into five individual layers and separated by the plugs of the organic phases in a nanopipette when the salt (THATPBCl) concentration in the organic phase was 100 mM. Second, the aspiration rate of the MP was stabilized and the velocity of the aqueous phase in the MP was lowered at higher THATPBCl concentrations in the organic phase. This was because the force during ingression of the aqueous phase into the organic - phase-filled nanopipette induced an electro-osmotic flow between the inside wall of the nanopipette and THATPBCl in the organic phase. Third, inclusion of mRNA barcoding tags in the MP facilitated complementary DNA construction and sequential analysis of gene expression. This technique has potential to be applicable to RNA sequencing from different cell samples across the life sciences. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)961-969
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume409
Issue number4
DOIs
Publication statusPublished - 2017

Keywords

  • Liquid/liquid interface
  • Microfluidics
  • PCR
  • mRNA

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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