Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice

Guohong Zhang, Xiaohong Huang, Damdinsuren Boldbaatar, Banzragch Battur, Badgar Battsetseg, Houshuang Zhang, Longzheng Yu, Yan Li, Yuzi Luo, Shinuo Cao, Youn Kyong Goo, Junya Yamagishi, Jinlin Zhou, Shoufa Zhang, Hiroshi Suzuki, Ikuo Igarashi, Takeshi Mikami, Yoshifumi Nishikawa, Xuenan Xuan

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine.

    Original languageEnglish
    Pages (from-to)7243-7247
    Number of pages5
    JournalVaccine
    Volume28
    Issue number45
    DOIs
    Publication statusPublished - 2010 Oct 21

    Keywords

    • Neospora caninum
    • Recombinant vaccine
    • TgSAG1
    • Toxoplasma gondii
    • Vector

    ASJC Scopus subject areas

    • Molecular Medicine
    • Immunology and Microbiology(all)
    • veterinary(all)
    • Public Health, Environmental and Occupational Health
    • Infectious Diseases

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