Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome

S. Tabata; A. Higashitani; M. Takanami; K. Akiyama; Y. Kohara; Y. Nishimura; A. Nishimura; S. Yasuda; Y. Hirota

doi:10.1128/jb.171.2.1214-1218.1989

Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome

S. Tabata, A. Higashitani, M. Takanami, K. Akiyama, Y. Kohara, Y. Nishimura, A. Nishimura, S. Yasuda, Y. Hirota

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

Original language	English
Pages (from-to)	1214-1218
Number of pages	5
Journal	Journal of bacteriology
Volume	171
Issue number	2
DOIs	https://doi.org/10.1128/jb.171.2.1214-1218.1989
Publication status	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/jb.171.2.1214-1218.1989

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abstract = "A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.",

author = "S. Tabata and A. Higashitani and M. Takanami and K. Akiyama and Y. Kohara and Y. Nishimura and A. Nishimura and S. Yasuda and Y. Hirota",

year = "1989",

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AU - Tabata, S.

AU - Higashitani, A.

AU - Takanami, M.

AU - Akiyama, K.

AU - Kohara, Y.

AU - Nishimura, Y.

AU - Nishimura, A.

AU - Yasuda, S.

AU - Hirota, Y.

PY - 1989

Y1 - 1989

N2 - A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

AB - A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

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DO - 10.1128/jb.171.2.1214-1218.1989

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Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome

Abstract

ASJC Scopus subject areas

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