Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome

S. Tabata, A. Higashitani, M. Takanami, K. Akiyama, Y. Kohara, Y. Nishimura, A. Nishimura, S. Yasuda, Y. Hirota

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

Original languageEnglish
Pages (from-to)1214-1218
Number of pages5
JournalJournal of bacteriology
Volume171
Issue number2
DOIs
Publication statusPublished - 1989 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    Tabata, S., Higashitani, A., Takanami, M., Akiyama, K., Kohara, Y., Nishimura, Y., Nishimura, A., Yasuda, S., & Hirota, Y. (1989). Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. Journal of bacteriology, 171(2), 1214-1218. https://doi.org/10.1128/jb.171.2.1214-1218.1989