Abstract
A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.
| Original language | English |
|---|---|
| Pages (from-to) | 1214-1218 |
| Number of pages | 5 |
| Journal | Journal of bacteriology |
| Volume | 171 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1989 Jan 1 |
| Externally published | Yes |
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
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Cite this
Tabata, S., Higashitani, A., Takanami, M., Akiyama, K., Kohara, Y., Nishimura, Y., Nishimura, A., Yasuda, S., & Hirota, Y. (1989). Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. Journal of bacteriology, 171(2), 1214-1218. https://doi.org/10.1128/jb.171.2.1214-1218.1989