Construction of a thiamine pyrophosphate high-producing strain of Aspergillus oryzae by overexpression of three genes involved in thiamine biosynthesis

Misato Tokui, Takafumi Kubodera, Katsuya Gomi, Nobuo Yamashita, Akira Nishimura

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We have found a gene (thiP) encoding thiamine pyrophosphokinase (TPK) in the Aspergillus oryzae genome. No riboswitch-like region was found in the upstream region of thiP, although it was repressed probably by thiamine pyrophosphate (TPP) as well as thiA and nmtA, which are strictly regulated by TPP-riboswitch sequence. To improve the productivity of TPP in A. oryzae, we constructed the strain in which thiA, nmtA and thiP were overexpressed simultaneously. The resulting strain accumulated intracellular TPP 4-fold higher than did the control strain.

Original languageEnglish
Pages (from-to)388-390
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

Keywords

  • Aspergillus oryzae
  • Thiamine biosynthesis
  • Thiamine production
  • Thiamine pyrophosphate
  • Thiamine pyrophosphokinase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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