Construction of a 19F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry

Yedi Sun; Yousuke Takaoka; Shinya Tsukiji; Michiko Narazaki; Tetsuya Matsuda; Itaru Hamachi

doi:10.1016/j.bmcl.2011.06.038

Construction of a ¹⁹F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry

Yedi Sun, Yousuke Takaoka, Shinya Tsukiji, Michiko Narazaki, Tetsuya Matsuda, Itaru Hamachi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

In this study, assisted by affinity-guided DMAP strategy, we developed a novel ¹⁹F-modified lectin as a biosensor for specific detection and imaging of glycoproteins. Exploited the large chemical shift anisotropy property of ¹⁹F nuclei, glycoproteins detected by our ¹⁹F- biosensor are signatured by broadened peaks in ¹⁹F NMR, hence enabled the distinction between glycoproteins and small molecule saccharides. Such signal on/off switching was also applied to glycoprotein imaging by ¹⁹F MRI.

Original language	English
Pages (from-to)	4393-4396
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	21
Issue number	15
DOIs	https://doi.org/10.1016/j.bmcl.2011.06.038
Publication status	Published - 2011 Aug 1
Externally published	Yes

Keywords

Biosensor
F NMR/MRI
Glycosylation
Protein labeling
Protein-Protein interaction

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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title = "Construction of a 19F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry",

abstract = "In this study, assisted by affinity-guided DMAP strategy, we developed a novel 19F-modified lectin as a biosensor for specific detection and imaging of glycoproteins. Exploited the large chemical shift anisotropy property of 19F nuclei, glycoproteins detected by our 19F- biosensor are signatured by broadened peaks in 19F NMR, hence enabled the distinction between glycoproteins and small molecule saccharides. Such signal on/off switching was also applied to glycoprotein imaging by 19F MRI.",

keywords = "Biosensor, F NMR/MRI, Glycosylation, Protein labeling, Protein-Protein interaction",

author = "Yedi Sun and Yousuke Takaoka and Shinya Tsukiji and Michiko Narazaki and Tetsuya Matsuda and Itaru Hamachi",

note = "Funding Information: Y.S. and Y.T. acknowledge JSPS Research Fellowshios for Young Scientists. This work was partly supported by the CK Integrated Medical Bioimaging Project (MEXT) and CREST (Japan Science and Technology Agency). ",

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AU - Sun, Yedi

AU - Takaoka, Yousuke

AU - Tsukiji, Shinya

AU - Narazaki, Michiko

AU - Matsuda, Tetsuya

AU - Hamachi, Itaru

N1 - Funding Information: Y.S. and Y.T. acknowledge JSPS Research Fellowshios for Young Scientists. This work was partly supported by the CK Integrated Medical Bioimaging Project (MEXT) and CREST (Japan Science and Technology Agency).

PY - 2011/8/1

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