Construction of a 19F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry

Yedi Sun, Yousuke Takaoka, Shinya Tsukiji, Michiko Narazaki, Tetsuya Matsuda, Itaru Hamachi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

In this study, assisted by affinity-guided DMAP strategy, we developed a novel 19F-modified lectin as a biosensor for specific detection and imaging of glycoproteins. Exploited the large chemical shift anisotropy property of 19F nuclei, glycoproteins detected by our 19F- biosensor are signatured by broadened peaks in 19F NMR, hence enabled the distinction between glycoproteins and small molecule saccharides. Such signal on/off switching was also applied to glycoprotein imaging by 19F MRI.

Original languageEnglish
Pages (from-to)4393-4396
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume21
Issue number15
DOIs
Publication statusPublished - 2011 Aug 1
Externally publishedYes

Keywords

  • Biosensor
  • F NMR/MRI
  • Glycosylation
  • Protein labeling
  • Protein-Protein interaction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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