Abstract
In this study, assisted by affinity-guided DMAP strategy, we developed a novel 19F-modified lectin as a biosensor for specific detection and imaging of glycoproteins. Exploited the large chemical shift anisotropy property of 19F nuclei, glycoproteins detected by our 19F- biosensor are signatured by broadened peaks in 19F NMR, hence enabled the distinction between glycoproteins and small molecule saccharides. Such signal on/off switching was also applied to glycoprotein imaging by 19F MRI.
Original language | English |
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Pages (from-to) | 4393-4396 |
Number of pages | 4 |
Journal | Bioorganic and Medicinal Chemistry Letters |
Volume | 21 |
Issue number | 15 |
DOIs | |
Publication status | Published - 2011 Aug 1 |
Externally published | Yes |
Keywords
- Biosensor
- F NMR/MRI
- Glycosylation
- Protein labeling
- Protein-Protein interaction
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry