Construction of a recombinant Trichoderma reesei strain expressing Aspergillus aculeatus β-glucosidase 1 for efficient biomass conversion

Hikaru Nakazawa, Tetsushi Kawai, Noriko Ida, Yosuke Shida, Yoshinori Kobayashi, Hirofumi Okada, Shuji Tani, Jun ichi Sumitani, Takashi Kawaguchi, Yasushi Morikawa, Wataru Ogasawara

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

To develop a Trichoderma reesei strain appropriate for the saccharification of pretreated cellulosic biomass, a recombinant T. reesei strain, X3AB1, was constructed that expressed an Aspergillus aculeatus β-glucosidase 1 with high specific activity under the control of the xyn3 promoter. The culture supernatant from T. reesei X3AB1 grown on 1% Avicel as a carbon source had 63- and 25-fold higher β-glucosidase activity against cellobiose compared to that of the parent strain PC-3-7 and that of the T. reesei recombinant strain expressing an endogenous β-glucosidase I, respectively. Further, the xylanase activity was 30% lower than that of PC-3-7 due to the absence of xyn3. X3AB1 grown on 1% Avicel-0.5% xylan medium produced 2.3- and 3.3-fold more xylanase and β-xylosidase, respectively, than X3AB1 grown on 1% Avicel. The supernatant from X3AB1 grown on Avicel and xylan saccharified NaOH-pretreated rice straw efficiently at a low enzyme dose, indicating that the strain has good potential for use in cellulosic biomass conversion processes.

Original languageEnglish
Pages (from-to)92-99
Number of pages8
JournalBiotechnology and Bioengineering
Volume109
Issue number1
DOIs
Publication statusPublished - 2012 Jan
Externally publishedYes

Keywords

  • Aspergillus aculeatus
  • Bioconversion
  • Cellulase
  • Trichoderma reesei
  • xyn3
  • β-glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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